Definition of constitutive and stage-enriched promoters in the rodent malaria parasite, Plasmodium yoelii.

ABSTRACT:

Background

Well-defined promoters are essential elements for genetic studies in all organisms, and enable controlled expression of endogenous genes, transgene expression, and gene editing. Despite this, there is a paucity of defined promoters for the rodent-infectious malaria parasites. This is especially true for Plasmodium yoelii, which is often used to study the mosquito and liver stages of malarial infection, as well as host immune responses to infection.

Methods

Here six promoters were selected from across the parasite's life cycle (clag-a, dynein heavy chain delta, lap4, trap, uis4, lisp2) that have been invoked in the literature as controlling their genes in a stage-specific manner. A minimal promoter length for the constitutive pybip promoter that confers strong expr

SUBMITTER: Bowman LM 

PROVIDER: S-EPMC7685602 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature