Systematic identification of cis-regulatory variants that cause gene expression differences in a yeast cross.

ABSTRACT: Sequence variation in regulatory DNA alters gene expression and shapes genetically complex traits. However, the identification of individual, causal regulatory variants is challenging. Here, we used a massively parallel reporter assay to measure the cis-regulatory consequences of 5832 natural DNA variants in the promoters of 2503 genes in the yeast Saccharomyces cerevisiae. We identified 451 causal variants, which underlie genetic loci known to affect gene expression. Several promoters harbored multiple causal variants. In five promoters, pairs of variants showed non-additive, epistatic interactions. Causal variants were enriched at conserved nucleotides, tended to have low derived allele frequency, and were depleted from promoters of essential genes, which is consistent with

SUBMITTER: Renganaath K 

PROVIDER: S-EPMC7685706 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature