Extended field-of-view ultrathin microendoscopes for high-resolution two-photon imaging with minimal invasiveness.

ABSTRACT: Imaging neuronal activity with high and homogeneous spatial resolution across the field-of-view (FOV) and limited invasiveness in deep brain regions is fundamental for the progress of neuroscience, yet is a major technical challenge. We achieved this goal by correcting optical aberrations in gradient index lens-based ultrathin (≤500 µm) microendoscopes using aspheric microlenses generated through 3D-microprinting. Corrected microendoscopes had extended FOV (eFOV) with homogeneous spatial resolution for two-photon fluorescence imaging and required no modification of the optical set-up. Synthetic calcium imaging data showed that, compared to uncorrected endoscopes, eFOV-microendoscopes led to improved signal-to-noise ratio and more precise evaluation of correlated neuronal acti

SUBMITTER: Antonini A 

PROVIDER: S-EPMC7685710 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature