Ischemic Cerebral Endothelial Cell-Derived Exosomes Promote Axonal Growth.

ABSTRACT:

Background and purpose

Cerebral endothelial cells (CECs) and axons of neurons interact to maintain vascular and neuronal homeostasis and axonal remodeling in normal and ischemic brain, respectively. However, the role of exosomes in the interaction of CECs and axons in brain under normal conditions and after stroke is unknown.

Methods

Exosomes were isolated from CECs of nonischemic rats and is chemic rats (nCEC-exos and isCEC-exos), respectively. A multicompartmental cell culture system was used to separate axons from neuronal cell bodies.

Results

Axonal application of nCEC-exos promotes axonal growth of cortical neurons, whereas isCEC-exos further enhance axonal growth than nCEC-exos. Ultrastructural analysis revealed that CEC-exos applied into distal axons were inter

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7686085 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature