Epithelium-derived IL-33 activates mast cells to initiate neutrophil recruitment following corneal injury.

ABSTRACT:

Purpose

Neutrophils play a critical role in defending against threats such as microbial infection, yet their activation during innate immune response incites collateral damage to healthy tissues. We have previously shown that corneal injury induces mast cells to express the neutrophil chemoattractant CXCL2. Here we delineate the mechanism of injury-induced, non-IgE-mediated mast cell activation at the ocular surface.

Methods

Corneal injury was induced by mechanical removal of the epithelium and anterior stroma in mast cell deficient (cKit^W-sh) and C57BL/6 mice using Algerbrush II. Corneas were analyzed for frequencies of total CD45⁺ inflammatory cells, CD11b⁺Ly6G⁺ neutrophils, and cKit⁺FcεR1⁺ mast cells using

SUBMITTER: Elbasiony E 

PROVIDER: S-EPMC7686153 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature