Activation of ocular surface mast cells promotes corneal neovascularization.

ABSTRACT:

Purpose

Mast cells, historically known for their effector function in the induction of allergic diseases, reside in all vascularized tissues of the body in particular proximity to blood and lymphatic vessels. As neighboring sentinel cells to blood vessels, mast cells have been associated with angiogenesis. Here we assess the direct contribution of mast cells to neovascularization at the ocular surface.

Methods

Corneal neovascularization was induced by placing a single figure-of-eight intrastromal suture 1 mm from the limbus in mast cell-deficient (cKit^W-sh), C57BL/6, and Balb/c mice. Corneas were harvested at 6 h post-suture to quantify cKit⁺FcεR1⁺ mast cells using flow cytometry and tear wash was collected within 6 h to measure β-hexosaminid

SUBMITTER: Cho W 

PROVIDER: S-EPMC7686271 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature