Targeting T-cell oxidative metabolism to improve influenza survival in a mouse model of obesity.

ABSTRACT:

Background

Obesity is associated with impaired primary and secondary immune responses to influenza infection, with T cells playing a critical role. T-cell function is highly influenced by the cellular metabolic state; however, it remains unknown how altered systemic metabolism in obesity alters T-cell metabolism and function to influence immune response. Our objective was to identify the altered cellular metabolic state of T cells from obese mice so that we may target T-cell metabolism to improve immune response to infection.

Methods

Mice were fed normal chow or high-fat diet for 18-19 weeks. Changes in T-cell populations were analyzed in both adipose tissue and spleens using flow cytometry. Splenic T cells were further analyzed for nutrient uptake and extracellular metaboli

SUBMITTER: Alwarawrah Y 

PROVIDER: S-EPMC7686301 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature