Cyclical aggregation extends in vitro expansion potential of human mesenchymal stem cells.

ABSTRACT: Mesenchymal stem cell (MSC)-based therapy has shown great promises in various animal disease models. However, this therapeutic potency has not been well claimed when applied to human clinical trials. This is due to both the availability of MSCs at the time of administration and lack of viable expansion strategies. MSCs are very susceptible to in vitro culture environment and tend to adapt the microenvironment which could lead to cellular senescence and aging. Therefore, extended in vitro expansion induces loss of MSC functionality and its clinical relevance. To combat this effect, this work assessed a novel cyclical aggregation as a means of expanding MSCs to maintain stem cell functionality. The cyclical aggregation consists of an aggregation phase and an expansion phase by replating the

SUBMITTER: Bijonowski BM 

PROVIDER: S-EPMC7686385 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature