Establishment of a duplex real-time qPCR method for detection of Salmonella spp. and Serratia fonticola in fishmeal.

ABSTRACT: Salmonella spp. is a high-risk bacterial pathogen that is monitored in imported animal-derived feedstuffs. Serratia fonticola is the bacterial species most frequently confused with Salmonella spp. in traditional identification methods based on biochemical characteristics, which are time-consuming and labor-intensive, and thus unsuitable for daily inspection and quarantine work. In this study, we established a duplex real-time qPCR method with invA- and gyrB-specific primers and probes corresponding to Salmonella spp. and S. fonticola. The method could simultaneously detect both pathogens in imported feedstuffs, with a minimum limit of detection for Salmonella spp. and S. fonticola of 197 copies/μL and 145 copies/μL, respectively (correlation coefficient R² = 0.999 in both cases)

SUBMITTER: Ruan J 

PROVIDER: S-EPMC7686437 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature