Genome-wide transcriptomic analysis of microglia reveals impaired responses in aged mice after cerebral ischemia.

ABSTRACT: Senescence-associated alterations in microglia may have profound impact on cerebral homeostasis and stroke outcomes. However, the lack of a transcriptome-wide comparison between young and aged microglia in the context of ischemia limits our understanding of aging-related mechanisms. Herein, we performed RNA sequencing analysis of microglia purified from cerebral hemispheres of young adult (10-week-old) and aged (18-month-old) mice five days after distal middle cerebral artery occlusion or after sham operation. Considerable transcriptional differences were observed between young and aged microglia in healthy brains, indicating heightened chronic inflammation in aged microglia. Following stroke, the overall transcriptional activation was more robust (>13-fold in the number of genes upregulat

SUBMITTER: Shi L 

PROVIDER: S-EPMC7687039 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature