Effect of structural stability on endolysosomal degradation and T-cell reactivity of major shrimp allergen tropomyosin.

ABSTRACT:

Background

Tropomyosins are highly conserved proteins, an attribute that forms the molecular basis for their IgE antibody cross-reactivity. Despite sequence similarities, their allergenicity varies greatly between ingested and inhaled invertebrate sources. In this study, we investigated the relationship between the structural stability of different tropomyosins, their endolysosomal degradation patterns, and T-cell reactivity.

Methods

We investigated the differences between four tropomyosins-the major shrimp allergen Pen m 1 and the minor allergens Der p 10 (dust mite), Bla g 7 (cockroach), and Ani s 3 (fish parasite)-in terms of IgE binding, structural stability, endolysosomal degradation and subsequent peptide generation, and T-cell cross-reactivity in a BALB/c murine model

SUBMITTER: Kamath SD 

PROVIDER: S-EPMC7687109 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature