Deconvolution of cellular subsets in human tissue based on targeted DNA methylation analysis at individual CpG sites.

ABSTRACT:

Background

The complex composition of different cell types within a tissue can be estimated by deconvolution of bulk gene expression profiles or with various single-cell sequencing approaches. Alternatively, DNA methylation (DNAm) profiles have been used to establish an atlas for multiple human tissues and cell types. DNAm is particularly suitable for deconvolution of cell types because each CG dinucleotide (CpG site) has only two states per DNA strand-methylated or non-methylated-and these epigenetic modifications are very consistent during cellular differentiation. So far, deconvolution of DNAm profiles implies complex signatures of many CpGs that are often measured by genome-wide analysis with Illumina BeadChip microarrays. In this study, we investigated if the characterization

SUBMITTER: Schmidt M 

PROVIDER: S-EPMC7687708 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature