Hepatocyte high-mobility group box 1 protects against steatosis and cellular stress during high fat diet feeding.

ABSTRACT:

Background

Circulating high-mobility group box 1 (HMGB1) plays important roles in the pathogenesis of nonalcoholic fatty liver disease (NAFLD). Intracellular HMGB1 is critical for the biology of hepatocytes. However, the intracellular role of HMGB1 in hepatocellular steatosis is unknown. Therefore, we aimed to investigate the role of hepatocyte-specific HMGB1 (HC-HMGB1) in development of hepatic steatosis.

Methods

Wild type (WT) C57BL/6 and HC-HMGB1^-/- mice were fed high-fat diet (HFD) or low-fat diet (LFD) for up to 16 weeks.

Results

As expected, HMGB1 translocated from nuclear into cytoplasm and released into circulation after HFD treatment. HC-HMGB1 deficiency significantly reduced circulating HMGB1, suggesting that hepatocyte is a major source of circul

SUBMITTER: Lin M 

PROVIDER: S-EPMC7687718 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature