Improving cell survival and engraftment in vivo via layer-by-layer nanocoating of hESC-derived RPE cells.

ABSTRACT:

Background

Human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cell transplants have served as a cell therapy for treating retinal degenerative diseases. However, how to optimize the survival and engraftment of hESC-RPE cells is a great challenge.

Methods

Here, we report hESC-RPE cells that are embedded with polyelectrolytes gelatin and alginate by layer-by-layer (LbL) self-assembly technique, based on the opposite charge of alternate layers. Cells were assessed for cell survival, immunogenicity, and function in vitro and in vivo.

Results

This strategy obviously decreased the immunogenicity of hESC-RPE cells without affecting its activity. LbL-RPE cell transplants into the subretinal space of Royal College of Surgeons (RCS) rats optimized cell engr

SUBMITTER: Ru L 

PROVIDER: S-EPMC7687756 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature