CloneSifter: enrichment of rare clones from heterogeneous cell populations.

ABSTRACT:

Background

Many biological processes, such as cancer metastasis, organismal development, and acquisition of resistance to cytotoxic therapy, rely on the emergence of rare sub-clones from a larger population. Understanding how the genetic and epigenetic features of diverse clones affect clonal fitness provides insight into molecular mechanisms underlying selective processes. While large-scale barcoding with NGS readout has facilitated cellular fitness assessment at the population level, this approach does not support characterization of clones prior to selection. Single-cell genomics methods provide high biological resolution, but are challenging to scale across large populations to probe rare clones and are destructive, limiting further functional analysis of important clones.

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SUBMITTER: Feldman D 

PROVIDER: S-EPMC7687773 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature