Label-free quantitative proteomic analysis of insect larval and metamorphic molts.

ABSTRACT:

Background

Molting is an essential biological process occurring characteristic times throughout the life cycle of holometabolous insects. However, it is not clear how insects determine the direction of molting to remain status quo or to initiate metamorphosis. To explore the functional factors that determine the direction of molts, liquid chromatography-mass spectrometry was used to identify the molecules involved in larval and metamorphic molting, and the differentially expressed proteins (DEPs) were compared in the two processes.

Results

There were 321 and 1140 DEPs identified in larval and metamorphic molting process, respectively. Bioinformatics analyses show that the amino sugar pathway was up-regulated in both processes. The up-regulated protease contributed to the met

SUBMITTER: Si W 

PROVIDER: S-EPMC7687791 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature