System-wide molecular dynamics of endothelial dysfunction in Gram-negative sepsis.

ABSTRACT:

Background

Inflammation affecting whole organism vascular networks plays a central role in the progression and establishment of several human diseases, including Gram-negative sepsis. Although the molecular mechanisms that control inflammation of specific vascular beds have been partially defined, knowledge lacks on the impact of these on the molecular dynamics of whole organism vascular beds. In this study, we have generated an in vivo model by coupling administration of lipopolysaccharide with stable isotope labeling in mammals to mimic vascular beds inflammation in Gram-negative sepsis and to evaluate its effects on the proteome molecular dynamics. Proteome molecular dynamics of individual vascular layers (glycocalyx (GC), endothelial cells (EC), and smooth muscle cells (SMC)) w

SUBMITTER: Gallart-Palau X 

PROVIDER: S-EPMC7687804 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature