Drosophila sperm development and intercellular cytoplasm sharing through ring canals do not require an intact fusome.

ABSTRACT: Animal germ cells communicate directly with each other during gametogenesis through intercellular bridges, often called ring canals (RCs), that form as a consequence of incomplete cytokinesis during cell division. Developing germ cells in Drosophila have an additional specialized organelle connecting the cells called the fusome. Ring canals and the fusome are required for fertility in Drosophila females, but little is known about their roles during spermatogenesis. With live imaging, we directly observe the intercellular movement of GFP and a subset of endogenous proteins through RCs during spermatogenesis, from two-cell diploid spermatogonia to clusters of 64 post-meiotic haploid spermatids, demonstrating that RCs are stable and open to intercellular traffic throughout sperm

SUBMITTER: Kaufman RS 

PROVIDER: S-EPMC7687857 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature