The H741D mutation in Tac1p contributes to the upregulation of CDR1 and CDR2 expression in Candida albicans.

ABSTRACT: The wide use of antifungal agents has led to the development of resistance in the pathogenic yeast strain Candida albicans. Gain-of-function mutations in transcription factors such as Tac1p demonstrated their ability to control expression of the ABC transporter genes CDR1 and CDR2, and mediation of azole resistance. Previously, we obtained a series of azole-resistant isolates with high-level expression of CDR1 or/and CDR2, and identified the novel H741D mutation in Tac1p. In the present study, the TAC1 alleles from isolate C13 were introduced into tac1Δ/Δ mutant. The H741D change was seen in TAC1^C13 in addition to several other amino acid differences. Hyperactive alleles TAC1^C13 exhibited higher minimum inhibitory concentrations (MICs) of fluconazole and itraconazole

SUBMITTER: Liu JY 

PROVIDER: S-EPMC7688751 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature