Simultaneous blood and brain microdialysis in a free-moving mouse to test blood-brain barrier permeability of chemicals.

ABSTRACT: Neurotoxic chemicals that pass through the blood-brain barrier (BBB) can influence brain function. Efficient methods to test the permeability of the BBB to specific chemicals would facilitate identification of potentially neurotoxic agents. We report here a simultaneous blood and brain microdialysis in a free-moving mouse to test BBB permeability of different chemicals. Microdialysis sampling was conducted in mice at 3-5 days after implantation of a brain microdialysis probe and 1 day after implantation of a blood microdialysis probe. Therefore, mice were under almost physiological conditions. Results of an intravenous injection of lucifer yellow or uranine showed that the BBB was functioning in the mice under the experimental conditions. Mice were given phenyl arsenic compounds orally, an

SUBMITTER: Umezu T 

PROVIDER: S-EPMC7689036 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature