Rat Auditory Inner Hair Cell Mechanotransduction and Stereociliary Membrane Diffusivity Are Similarly Modulated by Calcium.

ABSTRACT: The lipid bilayer plays a pivotal role in force transmission to many mechanically-gated channels. We developed the technology to monitor membrane diffusivity in order to test the hypothesis positing that Ca²⁺ regulates open probability (P _o) of cochlear hair cell mechanotransduction (MET) channels via the plasma membrane. The stereociliary membrane was more diffusive (9x) than the basolateral membrane. Elevating intracellular Ca²⁺ buffering or lowering extracellular Ca²⁺ reduced stereociliary diffusivity and increased MET P _o. In contrast, prolonged depolarization increased stereociliary diffusivity and reduced MET P _o. No comparable effects were noted for soma measurements. Although MET channels are loc

SUBMITTER: George SS 

PROVIDER: S-EPMC7689183 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature