Caspase-3 Cleaves Extracellular Vesicle Proteins During Auditory Brainstem Development.

ABSTRACT: Sound localization requires extremely precise development of auditory brainstem circuits, the molecular mechanisms of which are largely unknown. We previously demonstrated a novel requirement for non-apoptotic activity of the protease caspase-3 in chick auditory brainstem development. Here, we used mass spectrometry to identify proteolytic substrates of caspase-3 during chick auditory brainstem development. These auditory brainstem caspase-3 substrates were enriched for proteins previously shown to be cleaved by caspase-3, especially in non-apoptotic contexts. Functional annotation analysis revealed that our caspase-3 substrates were also enriched for proteins associated with several protein categories, including proteins found in extracellular vesicles (EVs), membrane-bound nanoparticles

SUBMITTER: Weghorst F 

PROVIDER: S-EPMC7689216 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature