Molecular epidemiology of resistance to antimalarial drugs in the Greater Mekong subregion: an observational study.

ABSTRACT:

Background

The Greater Mekong subregion is a recurrent source of antimalarial drug resistance in Plasmodium falciparum malaria. This study aimed to characterise the extent and spread of resistance across this entire region between 2007 and 2018.

Methods

P falciparum isolates from Myanmar, Thailand, Laos, and Cambodia were obtained from clinical trials and epidemiological studies done between Jan 1, 2007, and Dec 31, 2018, and were genotyped for molecular markers (pfkelch, pfcrt, pfplasmepsin2, and pfmdr1) of antimalarial drug resistance. Genetic relatedness was assessed using microsatellite and single nucleotide polymorphism typing of flanking sequences around target genes.

Findings

10 632 isolates were genotyped. A single long pfkelch Cys580Tyr haplotype (from -50 kb

SUBMITTER: Imwong M 

PROVIDER: S-EPMC7689289 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature