Characterization of glutathione proteome in CHO cells and its relationship with productivity and cholesterol synthesis.

ABSTRACT: Glutathione (GSH) plays a central role in the redox balance maintenance in mammalian cells. Previous studies of industrial Chinese hamster ovary cell lines have demonstrated a relationship between GSH metabolism and clone productivity. However, a thorough investigation is required to understand this relationship and potentially highlight new targets for cell engineering. In this study, we have modulated the GSH intracellular content of an industrial cell line under bioprocess conditions to further elucidate the role of the GSH synthesis pathway. Two strategies were used: the variation of cystine supply and the direct inhibition of the GSH synthesis using buthionine sulfoximine (BSO). Over time of the bioprocess, a correlation between intracellular GSH and product titer has been observed. A

SUBMITTER: Chevallier V 

PROVIDER: S-EPMC7689765 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature