Probing SWATH-MS as a tool for proteome level quantification in a nonmodel fish.

ABSTRACT: Quantitative proteomics via mass spectrometry can provide valuable insight into molecular and phenotypic characteristics of a living system. Recent mass spectrometry developments include data-independent acquisition (SWATH/DIA-MS), an accurate, sensitive and reproducible method for analysing the whole proteome. The main requirement for this method is the creation of a comprehensive spectral library. New technologies have emerged producing larger and more accurate species-specific libraries leading to a progressive collection of proteome references for multiple molecular model species. Here, for the first time, we set out to compare different spectral library constructions using multiple tissues from a coral reef fish to demonstrate its value and feasibility for nonmodel organisms. We creat

SUBMITTER: Monroe AA 

PROVIDER: S-EPMC7689905 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature