A New High-Throughput Screening Method to Detect Antimicrobial Volatiles from Metagenomic Clone Libraries.

ABSTRACT: The ever-growing spread of resistance in medicine and agriculture highlights the need to identify new antimicrobials. Microbial volatile organic compounds (VOCs) are one of the most promising groups of chemicals to meet this need. These rarely exploited molecules exhibit antimicrobial activity and their high vapour pressure makes them ideal for application in surface sterilisation, and in particular, in biofumigation. Therefore, we adapted the previously developed Two Clamp VOCs Assay (TCVA) to a new high-throughput screening for the detection of novel antifungal VOCs from metagenomic clone libraries. As a proof of concept, we tested the new high-throughput TCVA (htTCVA) by sourcing a moss metagenomic library against Fusarium culmorum. This led to the identification of five clones t

SUBMITTER: Stocker F 

PROVIDER: S-EPMC7690600 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature