A Novel Bead-Based Immunoassay for the Measurement of Heat Shock Proteins 27 and 70.

ABSTRACT: Heat shock proteins (HSPs) play an essential role in protecting proteins from denaturation and are implicated in diverse pathophysiological conditions like cardiovascular diseases, cancer, infections, and neurodegenerative diseases. Scientific evidence indicates that if HSP expression falls below a certain level, cells become sensitive to oxidative damage that accelerates protein aggregation diseases. On the other hand, persistently enhanced levels of HSP can lead to inflammatory and oncogenic changes. To date, although techniques for measuring HSPs exist, these assays are limited for use in specific sample types or are time consuming. Therefore, in the present study, we developed a single-molecule assay digital ELISA technology (Single Molecule Array-SIMOA) for the measurement of HSPs, wh

SUBMITTER: Njemini R 

PROVIDER: S-EPMC7690633 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature