Plasma Membrane Protein Nce102 Modulates Morphology and Function of the Yeast Vacuole.

ABSTRACT: Membrane proteins are targeted not only to specific membranes in the cell architecture, but also to distinct lateral microdomains within individual membranes to properly execute their biological functions. Yeast tetraspan protein Nce102 has been shown to migrate between such microdomains within the plasma membrane in response to an acute drop in sphingolipid levels. Combining microscopy and biochemistry methods, we show that upon gradual ageing of a yeast culture, when sphingolipid demand increases, Nce102 migrates from the plasma membrane to the vacuole. Instead of being targeted for degradation it localizes to V-ATPase-poor, i.e., ergosterol-enriched, domains of the vacuolar membrane, analogous to its plasma membrane localization. We discovered that, together with its homologue Fhn1, Nce

SUBMITTER: Vaskovicova K 

PROVIDER: S-EPMC7690685 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature