Prospects and limitations of expansion microscopy in chromatin ultrastructure determination.

ABSTRACT: Expansion microscopy (ExM) is a method to magnify physically a specimen with preserved ultrastructure. It has the potential to explore structural features beyond the diffraction limit of light. The procedure has been successfully used for different animal species, from isolated macromolecular complexes through cells to tissue slices. Expansion of plant-derived samples is still at the beginning, and little is known, whether the chromatin ultrastructure becomes altered by physical expansion. In this study, we expanded isolated barley nuclei and compared whether ExM can provide a structural view of chromatin comparable with super-resolution microscopy. Different fixation and denaturation/digestion conditions were tested to maintain the chromatin ultrastructure. We achieved up to ~4.2-times ph

SUBMITTER: Kubalova I 

PROVIDER: S-EPMC7691311 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature