RasV12 Expression in Microglia Initiates Retinal Inflammation and Induces Photoreceptor Degeneration.

ABSTRACT:

Purpose

The role of activated retinal microglia in driving retinal degeneration has been implicated in a number of in vivo disease models. Here, we investigated the primary consequences of microglial activation by the specific expression of constitutively active Ras in microglia in a transgenic mouse model before the onset of any degenerative changes in the retina.

Methods

The double transgenic lines CAG-LSL-RasV12-IRES-EGFP; Cx3cr1CreER/+ (Cx3cr1-RasV12 mice) and CAG-LSL-EGFP; Cx3cr1CreER_+ (control mice) were generated. The expression of RasV12 was induced in microglia by tamoxifen administration, and the retinas were examined by immunohistochemistry of frozen sections, RT-qPCR, and live imaging.

Results

RasV12 expression in retinal microglial cells promoted cell pr

SUBMITTER: Moriuchi Y 

PROVIDER: S-EPMC7691791 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature