Molecular Identification of Zoonotic Parasites of the Genus Anisakis (Nematoda: Anisakidae) from Fish of the Southeastern Pacific Ocean (Off Peru Coast).

ABSTRACT: The study aims to perform, for the first time, the molecular identification of anisakid larvae in commercial fish from the Southeastern Pacific Ocean off the Peru coast, and to provide data on their infection level by fishing ground, fish host, and site of infection. Fish specimens (N = 348) from the northern and the central coast of Peru were examined for parasites. The fish fillets were examined by the UV-press method. Anisakis spp. larvae (N = 305) were identified by mtDNA cox2 sequences analysis and by the ARMS-PCR of the locus nas10 nDNA. Two hundred and eighty-eight Anisakis Type I larvae corresponded to Anisakis pegreffii, whereas 17 Anisakis Type II larvae clustered in a phylogenetic lineage distinct from Anisakis physeteris deposited in GenBank, and corresponding to a phylogenetic

SUBMITTER: Aco Alburqueque R 

PROVIDER: S-EPMC7692232 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature