Blood Vessel Imaging at Pre-Larval Stages of Zebrafish Embryonic Development.

ABSTRACT: The zebrafish (Danio rerio) is an increasingly popular animal model biological system. In cardiovascular research, it has been used to model specific cardiac phenomena as well as to identify novel therapies for human cardiovascular disease. While the zebrafish cardiovascular system functioning is well examined at larval stages, the mechanisms by which vessel activity is initiated remain a subject of intense investigation. In this research, we report on an in vivo stain-free blood vessel imaging technique at pre-larval stages of zebrafish embryonic development. We have developed the algorithm for the enhancement, alignment and spatiotemporal analysis of bright-field microscopy images of zebrafish embryos. It enables the detection, mapping and quantitative characterization of cardiac activit

SUBMITTER: Machikhin AS 

PROVIDER: S-EPMC7692510 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature