Molecular Assessment of Chelonid Alphaherpesvirus 5 Infection in Tumor-Free Green (Chelonia mydas) and Loggerhead (Caretta caretta) Sea Turtles in North Carolina, USA, 2015-2019.

ABSTRACT: Fibropapillomatosis is associated with chelonid alphaherpesvirus 5 (ChHV5) and tumor formation in sea turtles. We collected blood samples from 113 green (Chelonia mydas) and 112 loggerhead (Caretta caretta) turtles without fibropapillomatosis, including 46 free-ranging turtles (20 green turtles, 26 loggerheads), captured in Core Sound, North Carolina, and 179 turtles (93 green turtles, 86 loggerheads) in rehabilitative care in North Carolina. Blood samples were analyzed for ChHV5 DNA using quantitative polymerase chain reaction (qPCR), and for antibodies to ChHV5 peptides using an enzyme-linked immunosorbent assay (ELISA). None of the samples from foraging turtles tested positive for ChHV5 by qPCR; ELISA was not used for foraging turtles. Samples from 18/179 (10.1%) rehabilitating turtles

SUBMITTER: Page-Karjian A 

PROVIDER: S-EPMC7692682 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature