Differences in gynecologic tumor development in Amhr2-Cre mice with KRAS^G12D or KRAS^G12V mutations.

ABSTRACT: How different KRAS variants impact tumor initiation and progression in vivo has not been thoroughly examined. We hypothesize that the ability of either KRAS^G12D or KRAS^G12V mutations to initiate tumor formation is context dependent. Amhr2-Cre mice express Cre recombinase in tissues that develop into the fallopian tubes, uterus, and ovaries. We used these mice to conditionally express either the KRAS^G12V/+ or KRAS^G12D/+ mutation. Mice with the genotype Amhr2-Cre Pten^(fl/fl) Kras^G12D/+(G12D mice) had abnormal follicle structures and developed low-grade serous ovarian carcinomas with 100% penetrance within 18 weeks. In contrast, mice with the genotype Amhr2-Cre Pten^(fl/fl) Kras^G12V/+ (G12V mice) had normal

SUBMITTER: Kun EHS 

PROVIDER: S-EPMC7693266 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature