Essential role of accessory subunit LYRM6 in the mechanism of mitochondrial complex I.

ABSTRACT: Respiratory complex I catalyzes electron transfer from NADH to ubiquinone (Q) coupled to vectorial proton translocation across the inner mitochondrial membrane. Despite recent progress in structure determination of this very large membrane protein complex, the coupling mechanism is a matter of ongoing debate and the function of accessory subunits surrounding the canonical core subunits is essentially unknown. Concerted rearrangements within a cluster of conserved loops of central subunits NDUFS2 (β1-β2^S2 loop), ND1 (TMH5-6^ND1 loop) and ND3 (TMH1-2^ND3 loop) were suggested to be critical for its proton pumping mechanism. Here, we show that stabilization of the TMH1-2^ND3 loop by accessory subunit LYRM6 (NDUFA6) is pivotal for energy conversion by mi

SUBMITTER: Galemou Yoga E 

PROVIDER: S-EPMC7693276 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature