The Landscape of Pseudomonas aeruginosa Membrane-Associated Proteins.

ABSTRACT:

Background

Pseudomonas aeruginosa cell envelope-associated proteins play a relevant role in infection mechanisms. They can contribute to the antibiotic resistance of the bacterial cells and be involved in the interaction with host cells. Thus, studies contributing to elucidating these key molecular elements are of great importance to find alternative therapeutics.

Methods

Proteins and peptides were extracted by different methods and analyzed by Multidimensional Protein Identification Technology (MudPIT) approach. Proteomic data were processed by Discoverer2.1 software and multivariate statistics, i.e., Linear Discriminant Analysis (LDA), while the Immune Epitope Database (IEDB) resources were used to predict antigenicity and immunogenicity of experimental identified p

SUBMITTER: Motta S 

PROVIDER: S-EPMC7694347 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature