An Unbiased Approach to Mapping the Signaling Network of the Pseudorabies Virus US3 Protein.

ABSTRACT: The US3 serine/threonine protein kinase is conserved among the alphaherpesvirus family and represents an important virulence factor. US3 plays a role in viral nuclear egress, induces dramatic alterations of the cytoskeleton, represses apoptosis, enhances gene expression and modulates the immune response. Although several substrates of US3 have been identified, an unbiased screen to identify US3 phosphorylation targets has not yet been described. Here, we perform a shotgun and phosphoproteomics analysis of cells expressing the US3 protein of pseudorabies virus (PRV) to identify US3 phosphorylation targets in an unbiased way. We identified several cellular proteins that are differentially phosphorylated upon US3 expression and validated the phosphorylation of lamin A/C at serine 404, both in

SUBMITTER: Jansens RJJ 

PROVIDER: S-EPMC7694389 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature