Chromosome End Diversification in Sciarid Flies.

ABSTRACT: Dipterans exhibit a remarkable diversity of chromosome end structures in contrast to the conserved system defined by telomerase and short repeats. Within dipteran families, structure of chromosome termini is usually conserved within genera. With the aim to assess whether or not the evolutionary distance between genera implies chromosome end diversification, this report exploits two representatives of Sciaridae, Rhynchosciara americana, and Trichomegalosphyspubescens. Probes and plasmid microlibraries obtained by chromosome end microdissection, in situ hybridization, cloning, and sequencing are among the methodological approaches employed in this work. The data argue for the existence of either specific terminal DNA sequences for each chromosome tip in T. pubescens, or sequences common to a

SUBMITTER: Gorab E 

PROVIDER: S-EPMC7694509 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature