A functional analysis of mitochondrial respiratory chain cytochrome bc₁ complex in Gaeumannomyces tritici by RNA silencing as a possible target of carabrone.

ABSTRACT: Gaeumannomyces tritici, an ascomycete soilborne fungus, causes a devastating root disease in wheat. Carabrone, a botanical bicyclic sesquiterpenic lactone, is a promising fungicidal agent that can effectively control G. tritici. However, the mechanism of action of carabrone against G. tritici remains largely unclear. Here, we used immunogold for subcellular localization of carabrone and the results showed that carabrone is subcellularly localized in the mitochondria of G. tritici. We then explored the functional analysis of genes GtCytc₁ , GtCytb, and GtIsp of the mitochondrial respiratory chain cytochrome bc₁ complex in G. tritici by RNA silencing as a possible target of carabrone. The results showed that the silenced mutant ∆GtIsp is less sensitive to carabrone comp

SUBMITTER: Wang M 

PROVIDER: S-EPMC7694678 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature