Effect of dilution of canine blood samples on the specificity of saline agglutination tests for immune-mediated hemolysis.

ABSTRACT:

Background

Saline agglutination tests (SATs) are widely recommended for diagnosis of immune-mediated hemolytic anemia in dogs, but there are frequent false-positive results.

Objectives

Specificity of SATs will improve at higher saline-to-blood ratios.

Animals

One hundred fifty dogs treated at a veterinary referral hospital with hematocrits ≤30%.

Methods

Prospective diagnostic accuracy study. Immune-mediated hemolysis (IMH) was considered present if a gel direct antiglobulin test (DAT) was positive and there was clinical evidence of hemolysis (n = 9), absent if another mechanism for anemia was identified and the DAT was negative or there was no hemolysis (n = 138), and if IMH status was unclear, dogs were excluded (n = 3). Saline agglutination tests were prepare

SUBMITTER: Sun PL 

PROVIDER: S-EPMC7694812 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature