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T Cells from NOD-PerIg Mice Target Both Pancreatic and Neuronal Tissue.

ABSTRACT: It has become increasingly appreciated that autoimmune responses against neuronal components play an important role in type 1 diabetes (T1D) pathogenesis. In fact, a large proportion of islet-infiltrating B lymphocytes in the NOD mouse model of T1D produce Abs directed against the neuronal type III intermediate filament protein peripherin. NOD-PerIg mice are a previously developed BCR-transgenic model in which virtually all B lymphocytes express the H and L chain Ig molecules from the intra-islet-derived anti-peripherin-reactive hybridoma H280. NOD-PerIg mice have accelerated T1D development, and PerIg B lymphocytes actively proliferate within islets and expand cognitively interactive pathogenic T cells from a pool of naive precursors. We now report adoptively transferred T cells or whole splenocytes from NOD-PerIg mice expectedly induce T1D in NOD.scid recipients but, depending on the kinetics of disease development, can also elicit a peripheral neuritis (with secondary myositis). This neuritis was predominantly composed of CD4⁺ and CD8⁺ T cells. Ab depletion studies showed neuritis still developed in the absence of NOD-PerIg CD8⁺ T cells but required CD4⁺ T cells. Surprisingly, sciatic nerve-infiltrating CD4⁺ cells had an expansion of IFN-γ^- and TNF-α^- double-negative cells compared with those within both islets and spleen. Nerve and islet-infiltrating CD4⁺ T cells also differed by expression patterns of CD95, PD-1, and Tim-3. Further studies found transitory early B lymphocyte depletion delayed T1D onset in a portion of NOD-PerIg mice, allowing them to survive long enough to develop neuritis outside of the transfer setting. Together, this study presents a new model of peripherin-reactive B lymphocyte-dependent autoimmune neuritis.

SUBMITTER: Racine JJ

PROVIDER: S-EPMC7694871 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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T Cells from NOD-<i>PerIg</i> Mice Target Both Pancreatic and Neuronal Tissue.

Racine Jeremy J JJ Chapman Harold D HD Doty Rosalinda R Cairns Brynn M BM Hines Timothy J TJ Tadenev Abigail L D ALD Anderson Laura C LC Green Torrian T Dyer Meaghan E ME Wotton Janine M JM Bichler Zoë Z White Jacqueline K JK Ettinger Rachel R Burgess Robert W RW Serreze David V DV

Journal of immunology (Baltimore, Md. : 1950) 20200916 8

It has become increasingly appreciated that autoimmune responses against neuronal components play an important role in type 1 diabetes (T1D) pathogenesis. In fact, a large proportion of islet-infiltrating B lymphocytes in the NOD mouse model of T1D produce Abs directed against the neuronal type III intermediate filament protein peripherin. NOD-<i>PerIg</i> mice are a previously developed BCR-transgenic model in which virtually all B lymphocytes express the H and L chain Ig molecules from the int ...[more]

PMID: 32938729

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Project description:Aims/hypothesisTolerance induction in lymph nodes can be mediated by both haematopoietic cells (e.g. specific dendritic cells subsets) and by non-haematopoietic cells (e.g. lymph node stromal cells [LNSCs]) when they present peripheral tissue antigens to autoreactive T cells. LNSCs normally regulate T cell trafficking and survival and help to maintain peripheral tolerance by exerting immunosuppressive effects. However, whether autoimmunity can be associated with defective tolerogenic functions of LNSCs is unknown and studies aimed at characterising LNSCs in humans are lacking. We hypothesised that dysregulated T cell responses in pancreatic lymph nodes (PLNs) from donors with type 1 diabetes and from NOD mice may be associated with altered LNSC function.MethodsWe analysed PLNs from donors with type 1 diabetes and NOD mice for LNSC distribution and phenotype using flow cytometry. We assessed the expression of tolerance-related genes in different subsets of LNSCs from human donors, as well as in a population of dendritic cells enriched in autoimmune regulator (AIRE)+ cells and identified as HLA-DRhigh CD45low.ResultsThe relative frequency of different LNSC subsets was altered in both donors with type 1 diabetes and NOD mice, and both MHC class II and programmed death-ligand 1 (PD-L1) expression were upregulated in human type 1 diabetes. Tolerance-related genes showed similar expression profiles between mouse and human LNSCs at steady state but were generally upregulated in the context of human type 1 diabetes, while, at the same time, many such genes were downregulated in the AIRE-enriched dendritic cell population.Conclusion/interpretationOur study shows that LNSCs are substantially altered in type 1 diabetes, but, surprisingly, they exhibit an enhanced tolerogenic phenotype along with increased antigen-presenting potential, which may indicate an attempt to offset dendritic cell-related tolerogenic defects in tolerance. Thus, LNSCs could constitute alternative therapeutic targets in which to deliver antigens to help re-establish tolerance and prevent or treat type 1 diabetes.Data availabilityAll data generated or analysed during this study are included in the published article (and its online supplementary files). Biomark gene expression data were deposited on the Mendeley repository at https://data.mendeley.com/datasets/d9rdzdmvyf/1 . Any other raw datasets are available from the corresponding author on reasonable request. No applicable resources were generated or analysed during the current study.

Dataset Information

T Cells from NOD-PerIg Mice Target Both Pancreatic and Neuronal Tissue.

Publications

T Cells from NOD-<i>PerIg</i> Mice Target Both Pancreatic and Neuronal Tissue.

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