Comprehensive proteomic analysis of exosomes derived from human bone marrow, adipose tissue, and umbilical cord mesenchymal stem cells.

ABSTRACT:

Background

Mesenchymal stem cell (MSC)-derived exosomes have shown comprehensive application prospects over the years. Despite performing similar functions, exosomes from different origins present heterogeneous characteristics and components; however, the relative study remains scarce. Lacking of a valuable reference, researchers select source cells for exosome studies mainly based on accessibility and personal preference.

Methods

In this study, exosomes secreted by MSCs derived from different tissues were isolated, by ultracentrifugation, and proteomics analysis was performed. A total of 1014 proteins were detected using a label-free method.

Results

Bioinformatics analysis revealed their shared function in the extracellular matrix receptor. Bone marrow MSC-derived ex

SUBMITTER: Wang ZG 

PROVIDER: S-EPMC7694919 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature