Development of a Multiplex Bead Assay for the Detection of IgG Antibody Responses to Guinea Worm.

ABSTRACT: The success of the Guinea Worm (GW) Eradication Program over the past three decades has been tempered by the persistence of GW disease in a few African nations and the potential for a future resurgence in cases. Domestic dogs are now a major concern as a disease reservoir as large numbers of cases of canine GW disease are now reported each year, mainly along the Chari River in Chad. As a first step toward the development of a serologic assay for dogs, archived human plasma samples from dracunculiasis-positive donors from Togo were used to select adult female GW antigens for peptide sequencing and cloning. Eight protein sequences of interest were expressed as recombinant glutathione-S-transferase (GST) fusion proteins, and the most promising proteins were coupled to carboxylated micr

SUBMITTER: Priest JW 

PROVIDER: S-EPMC7695067 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature