Differential Expression Profiling Reveals Stress-Induced Cell Fate Divergence in Soybean Microspores.

ABSTRACT: Stress-induced microspore embryogenesis is a widely employed method to achieve homozygosity in plant breeding programs. However, the molecular mechanisms that govern gametophyte de- and redifferentiation are understood poorly. In this study, RNA-Seq was used to evaluate global changes across the microspore transcriptome of soybean (Glycine max [L.] Merrill) as a consequence of pretreatment low-temperature stress. Expression analysis revealed more than 20,000 differentially expressed genes between treated and control microspore populations. Functional enrichment illustrated that many of these genes (e.g., those encoding heat shock proteins and cytochrome P450s) were upregulated to maintain cellular homeostasis through the mitigation of oxidative damage. Moreover, transcripts corresponding t

SUBMITTER: Hale B 

PROVIDER: S-EPMC7695151 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature