Islet vascularization is regulated by primary endothelial cilia via VEGF-A-dependent signaling.

ABSTRACT: Islet vascularization is essential for intact islet function and glucose homeostasis. We have previously shown that primary cilia directly regulate insulin secretion. However, it remains unclear whether they are also implicated in islet vascularization. At eight weeks, murine Bbs4^-/-islets show significantly lower intra-islet capillary density with enlarged diameters. Transplanted Bbs4^-/- islets exhibit delayed re-vascularization and reduced vascular fenestration after engraftment, partially impairing vascular permeability and glucose delivery to β-cells. We identified primary cilia on endothelial cells as the underlying cause of this regulation, via the vascular endothelial growth factor-A (VEGF-A)/VEGF receptor 2 (VEGFR2) pathway. In vitro silencing of

SUBMITTER: Xiong Y 

PROVIDER: S-EPMC7695455 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature