Vascular Kv7 channels control intracellular Ca²⁺ dynamics in smooth muscle.

ABSTRACT: Voltage-gated Kv7 (or KCNQ) channels control activity of excitable cells, including vascular smooth muscle cells (VSMCs), by setting their resting membrane potential and controlling other excitability parameters. Excitation-contraction coupling in muscle cells is mediated by Ca²⁺ but until now, the exact role of Kv7 channels in cytosolic Ca²⁺ dynamics in VSMCs has not been fully elucidated. We utilised microfluorimetry to investigate the impact of Kv7 channel activity on intracellular Ca²⁺ levels and electrical activity of rat A7r5 VSMCs and primary human internal mammary artery (IMA) SMCs. Both, direct (XE991) and G protein coupled receptor mediated (vasopressin, AVP) Kv7 channel inhibition induced robust Ca²⁺ oscillations, which were significan

SUBMITTER: Tsai YM 

PROVIDER: S-EPMC7695684 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature