Establishment and characterization of a PCOS and a normal human granulosa cell line.

ABSTRACT: Oocyte maturation is an important phase in fertility and any disorder in this process could lead to infertility. The most common disorder during folliculogenesis is polycystic ovary syndrome (PCOS). Due to the secretive activity of granulosa cells (GCs), they play a vital role in folliculogenesis. Although scientists use various cellular and molecular methods to have a better understanding of the mechanism of these cells, some limitations still exist in GC culture such as low primary cell yield and proliferation capability. Therefore, immortalization of primary cells is an approach to overcome these limitations. In the current study, GCs were obtained from two females, one with PCOS and one with normal folliculogenesis. In the first stage, we established two human GC (hGC) lines by immorta

SUBMITTER: Hashemian Z 

PROVIDER: S-EPMC7695766 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature