HESC-derived immune suppressive dendritic cells induce immune tolerance of parental hESC-derived allografts.

ABSTRACT:

Background

With their inherent capability of unlimited self-renewal and unique potential to differentiate into functional cells of the three germ layers, human embryonic stem cells (hESCs) hold great potential in regenerative medicine. A major challenge in the application of hESC-based cell therapy is the allogeneic immune rejection of hESC-derived allografts.

Methods

We derived dendritic cell-like cells (DCLs) from wild type and CTLA4-Ig/PD-L1 knock-in hESCs, denoted WT DCLs and CP DCLs. The expression of DC-related genes and surface molecules was evaluated, as well as their DCL capacity to stimulate allogeneic T cells and induce regulatory T (Treg) cells in vitro. Using an immune system humanized mouse model, we investigated whether the adoptive transfer of CP DCLs can ind

SUBMITTER: Todorova D 

PROVIDER: S-EPMC7695963 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature