Evidence for liver and peripheral immune cells secreting tumor-suppressive extracellular vesicles in melanoma patients.

ABSTRACT:

Background

Before and after surgery melanoma patients harbor elevated levels of extracellular vesicles in plasma (pEV), suppressing tumor cell activity. However, due to technical reasons and lack of cell-specific biomarkers, their cellular origin remains obscure.

Methods

We mimicked the interaction of tumor cells with liver cells and PBMC in vitro, and compared newly secreted EV-associated miRNAs and protein factors with those detected in melanoma patient`s pEV.

Findings

Our results suggest that pEV from melanoma patients are secreted in part by residual or relapsing tumor cells, but also by liver and peripheral blood mononuclear cells (PBMC). Our approach identified factors that were seemingly associated either with tumor cell activity, or the counteracting immune sy

SUBMITTER: Lee JH 

PROVIDER: S-EPMC7695971 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature