Cell Type-Specific Adhesion and Migration on Laser-Structured Opaque Surfaces.

ABSTRACT: Cytocompatibility is essential for implant approval. However, initial in vitro screenings mainly include the quantity of adherent immortalized cells and cytotoxicity. Other vital parameters, such as cell migration and an in-depth understanding of the interaction between native tissue cells and implant surfaces, are rarely considered. We investigated different laser-fabricated spike structures using primary and immortalized cell lines of fibroblasts and osteoblasts and included quantification of the cell area, aspect ratio, and focal adhesions. Furthermore, we examined the three-dimensional cell interactions with spike topographies and developed a tailored migration assay for long-term monitoring on opaque materials. While fibroblasts and osteoblasts on small spikes retained their normal mo

SUBMITTER: Schaeske J 

PROVIDER: S-EPMC7696563 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature